ABSTRACT
The sea has always been one of the most important medium that humanity has used to travel and as an extensive precious source of food, especially for poor countries. However, this has caused several uncontrolled problems including, for example, the pollution of the sea and the exploitation of its resources, often altering the ecosystem in a compromising and irreversible way. Thus it becomes mandatory for the survival of all humanity to think and find new methodologies to prevent, recognize and solve these issues, some of these of truly extensive relevance including, for example, the presence of plastics and microplastics in the oceans or the phytoplankton species richness [1]. The quality of marine seawater is usually tested by means of physical, chemical and microbio- logical experiments, which are often unsuitable for online monitoring. In particular, fluorescence spectroscopy has proven to be an effective technique for characterizing and monitoring sources of pollution and in treatment works for controlling and optimizing processes [1]. Fluorescence consists in the release of light when molecules, called fluorophores, are excited with a high-energy light source. This technique has been used for its multiple advantages: it is fast, inexpensive, reagent-free, requires little sample preparation, is highly sensitive and non-invasive . Furthermore, fluorescence monitoring could furnish a rapid feedback, enabling studies with high spatial and temporal resolution. Since our aim was to (i) check the plankton wellness, and possibly (ii) identify micro-plastics, we are going to measure the autofluorescence of marine seawater. Plankton fluorescence is mainly due to chlorophyll with absorption bands at ≈ 430 and 470 nm and emission ones at ≈ 680 and 720 nm [2]. Moreover, plastics exhibit a background of autofluorescence when excited by UV/VIS radiation. Additives, e.g. pigments, and the variability in the compositions of plastics reflects in a broad VIS autofluorescence band peaked near 400 nm [3].